银杏叶提取物对大鼠机械通气所致肺损伤SOD、MDA的影响

Effect of EGB on SOD, MDA of ventilator-induced lung injury in rats

目的:研究银杏叶提取物对大鼠机械通气所致肺损伤超氧化物歧化酶(SOD)、丙二醛(MDA)的影响及机制。方法:将30只雄性SD大鼠随机分为3组:对照组(C组)、大潮气量组(H组)和银杏叶提取物组(E组)。H组和E组设置机械通气潮气量30 mL/kg,呼吸频率40次/min,I/E为1/3,呼气末正压为0 cmH2O,吸入氧浓度为21%。机械通气4 h后收集支气管肺泡灌洗液和血清测定SOD和MDA的水平,取肺组织测定肺湿/干重比(W/D),观察肺组织病理改变。结果:H组与C组相比,MDA、W/D水平均显著增高,SOD水平则显著降低;E组与H组相比,大鼠MDA、W/D水平均显著降低,SOD水平则显著升高。肺组织病理改变显示E组的病理损害明显比H组减轻。结论:银杏叶提取物能减轻肺水肿,减少机械通气所致的肺损伤。

Objective To investigate the effect of EGB on SOD, MDA of ventilator- induced lung injury in rats and its possible mechanisms. Methods Thirty male SD rats were randomly divided into 3 groups： control group （C group）, high tidal ventilation group （H group） and EGB group （E group）. The setting mechanical ventilation was VT = 30 mL/kg, RR = 40/min, I/E = 1/3, PEEP = 0 cm H2O and FiO2 = 21%. The bronchoalveolar lavage fluid （BLAF） and serum were obtained for determination of the levels of SOD and MDA at the end of 4 h mechanical ventilation. The Lungs were removed, and the wet-to-dry weight ratio （W/D） and pulmonary pathologic changes were measured. Results As compared with C group, W/D and the levels of MDA were significantly increased in H group, but the levels of SOD were reduced in H group. As compared with H group, W/D and the levels of MDA were significantly decreased in E group, but the levels of SOD were increased in E group. Pulmonary pathologic changes were alleviated in E group comparing with H group. Conclusion EGB injection may have a protective role against hyperoxia and induced pulmonary damage in rats.