Hsa-miR-181a在子宫内膜癌变过程中的表达及意义

Expression and significance of Hsa-miR-181a in endometrial carcinoma

目的:探讨hsa-miR-181a(miR-181a)在子宫内膜癌变过程中的表达及其意义。方法:选取甲醛固定石蜡包埋组织75例,其中正常子宫内膜13例,子宫内膜增生症18例,子宫内膜癌44例。提取总RNA,采用实时荧光定量PCR技术检测各组子宫内膜组织中miR-181a的表达量。结果:在甲醛固定石蜡包埋组织中可检测到miR-181a的表达。miR-181a在子宫内膜癌组织中的表达高于子宫内膜增生症,在子宫内膜增生症组织中的表达高于正常子宫内膜,差异均有统计学意义(P<0.05);miR-181a的表达与FIGO分期有关,差异有统计学意义(P<0.05)。结论:miR-181a在子宫内膜癌组织中表达上调,可能起到了癌基因的作用;miR-181a在子宫内膜癌变过程中的差异表达可能与子宫内膜癌的发生、发展有关。

Objective To explore the expression and significance of hsa-miR-181a（miR-181a） in canceration progression of endometrial carcinoma. Methods 3. total of 75 formalin-fixed paraffin-embedded tissue specimens were studied in this study, of which, 13 were normal endometrium, 18 were endometrial hyperplasia, 44 were endometrial carcinoma. After total RNA had been extracted, real-time PCR was applied to detect the expression level of miR-181a in endometrial tissue in each group. Results miR-181a expression in formalin-fixed paraffin-embedded tissue specimens can be detected. Expression of miR-181a in endometrial carcinoma was higher than that in endometrial hyperplasia, its expression in endometrial hyperplasia was also higher than that in normal endometrium, and the difference was statistically significant （P 〈 0.05）. The expression of miR-181a in endometrial carcinoma was associated with FIGO stages （P 〈 0.05）. Conclusion The up-regulation of miR- 181a expression in women with endometrial carcinoma may play the role of oncogenes. Abnormal expression of miR-181a is probably associated with the occurrence and development of endometrial carcinoma.