含白花蛇舌草血清对大鼠淋巴细胞和混合培养淋巴细胞增殖影响的实验研究

Effect of the Serum Containing Herba Hedyotis Diffusae on Proliferation of Rats Splenic Lymphocytes and Mixed Lymphocytes

目的:研究含白花蛇舌草(Spreading Hedvotis Herb,简称SH)血清对植物血凝素(PHA)诱导大鼠脾淋巴细胞增殖、混合淋巴细胞培养(简称MLR)淋巴细胞增殖及对淋巴细胞分泌白细胞介素-2(IL-2)和γ-干扰素(IFN-γ)的影响。方法:大鼠20只,随机分为5组,每组4只,分为SH低剂量(4 g/kg)、中剂量(16 g/kg)、高剂量(24 g/kg)组,含环孢素A(CsA)血清组,按相应药物灌胃,空白血清组用等量生理盐水灌胃。MTT法测定PHA诱导的大鼠脾淋巴细胞和MLR淋巴细胞增殖,ELISA法测定PHA诱导淋巴细胞分泌IL-2、IFN-γ含量。结果:SH高剂量组可抑制PHA诱导大鼠脾淋巴细胞增殖,SH高剂量组、含CsA血清组与空白血清组比较,差异均有显著性意义(P<0.05)。双向MLR体系中,各剂量组含SH血清均可抑制淋巴细胞增殖,其中SH高剂量组与空白血清组比较,差异有显著性意义(P<0.05);在单向MLR体系中,各剂量组含SH血清可调节淋巴细胞增殖。SH中剂量组、SH高剂量组血清抑制脾淋巴细胞增殖,与空白血清组比较,差异均有显著性意义(P<0.05)。含CsA血清组血清可抑制单向、双向MLR体系中淋巴细胞增殖,与空白血清组比较,差异均有显著性意义(P<0.05)。SH中剂量组、SH高剂量组、含CsA血清组血清均可显著抑制大鼠脾淋巴细胞分泌IL-2、IFN-γ含量,与空白血清组比较,差异均有显著性意义(P<0.05)。结论:SH能抑制淋巴细胞和混合培养淋巴细胞增殖,其作用机制可能与抑制淋巴细胞分泌IL-2、IFN-γ有关。

Objective= To study the effect of the serum containing Herba Hedyotis Diffusae （HHD） on splenic lymphocytes proliferation induced by phytohemagglutinin （PHA）and in in-vitro mixed lymphocyte reaction （MLR） system as well as the contents of interleukin-2（IL-2） and interferon gamma（IFN-γ）. Methods： Twenty rats were randomly divided into five groups, blank group, cyclosporin a（CsA, in the dosage of 15 mg/kg） group, and high（H）-, middle（M）-, Iow（L）-dose HHD groups （in the dosage of 4, 16 and 24g/kg, respectively）. The blank group was administrated with normal saline. Lymphocyte proliferation induced by PHA and lymphocyte proliferation in MLR system were detected by means of M-R- assay. The levels of IL-2 and IFN-γ were detected by enzyme linked immunosorbent assay（ELISA）. Results： The inhibition of lymphocyte proliferation in H group and CsA group was superior to that in the blank group（P〈0.05）. In two-way MLR system, all HHD groups could inhibit the proliferation of lymphocytes, the difference between H group and the blank group being significant （P〈 0.05）. In one-way MLR system, all HHD groups could regulate the proliferation of lymphocytes. The inhibition of splenic lymphocytes proliferation in H group and M group was superior to that in the blank group（P〈 0.05）. In two-way MLR system, the difference between H group and the blank group was significant（P〈 0.05）. In both one-way and two-way MLR system, the inhibition of lymphocyte proliferation in CsA group was superior to that in the blank group（P〈 0.05）. The inhibition of IL-2, IFN-γ secretion in H group , M group and CsA group was superior to that in the blank group（P〈0.05）. Conclusion： HHB can inhibit the proliferation of lymphocytes and the mixed lymphocytes, and the mechanism is related with the inhibition of IL-2 and IFN-γ by rat iymphocytes