参麦开肺散对降低肺纤维化小鼠胶原生成的影响

Effects of Shenmai Kaifei Powder on Reduction of Collagenation in Mice with Pulmonary Fibrosis

目的：利用博莱霉素诱导的肺纤维化小鼠模型研究参麦开肺散对胶原生成的影响。方法：将6～8周龄C57BL/6小鼠单次气管灌注浓度为2.5 mg·kg-1的博莱霉素，建立肺纤维化模型；24只小鼠随机平均分为预防组和治疗组，两组分别包括对照组、模型组和药物组。从小鼠肺组织病理切片、肺组织胶原含量、胶原基因表达量、细胞外基质基因表达量等4个方面研究参麦开肺散在缓解肺纤维化过程中对胶原生成的影响。结果：无论是预防组还是治疗组，对照组中小鼠肺组织均无明显病理变化，模型组小鼠肺泡结构破坏，炎症细胞浸润，并有大量以胶原为主的细胞外基质沉积，表现出了严重的纤维化病变。经过药物治疗，小鼠肺泡结构接近正常，且细胞外基质沉积现象明显缓解；实时定量PCR结果显示，模型组小鼠肺组织中胶原基因Col1a1、Col1a2、Col3a1表达量显著升高，喂食药物后，胶原基因表达量大幅度下调。此外，模型组中细胞外基质基因如Ctgf、Fn1、Sparc、Tgf-β1的表达量相对于对照组均有所升高，而喂食药物之后其表达量均有所下调。结论：参麦开肺散通过降低相关纤维化基因如Ctgf、Fn1、Sparc、Tgf-β1的表达而有效减少胶原的生成，缓解纤维化进程。

Objective：To study the effects of Shenmai Kaifei Powder on collagenation in bleomycin-induced mice models with pulmonary ifbrosis.Methods：6~8-week-old C57BL/6 mice were given intratracheal instillation of bleomycin with the concentration of 2.5 mg·kg-1 to induce models with pulmonary ifbrosis;24 mice were randomly divided into the prevention group and the treatment group,respectively including the control group,the model group and the drug group.Studied the effects of Shenmai Kaifei Powder on collagenation in relieving pulmonary fibrosis from 4 aspects：pathological section of the mouse lung tissue,collagen content of the lung tissue,collagen gene expression and extracellular matrix gene expression.Results：No matter in the the prevention group or the treatment group,the lung tissue of the control group showed no signiifcant pathological changes,while in the model group,there were damage of alveolar structure,inflammatory cell infiltration and a large amount of extracellular matrix deposition composed mainly of collagen,showing a severe fibrosis.After treatment,the mouse alveolar structure recovered to normal and extracellular matrix deposition was alleviated;the results of real-time quantitative PCR showed a signiifcant increase of Col1a1,Col1a2 and Col3a1 expressions in the lung tissue of the model group.After feeding drugs,collagen gene expression significantly decreased.In addition,the expressions of extracellular matrix genes such as Ctgf,Fn1,Sparc and Tgf- β1in the model group increased compared with those of the control group,while the expressions reduced after giving drugs. Conclusion：Shenmai Kaifei Powder can effectively reduce the formation of collagen by reducing the expressions of related ifbrosis genes such as Ctgf,Fn1,Sparc and Tgf-β1,slowing the process of ifbrosis.