鸡产气荚膜梭菌的分离鉴定及药敏试验

Isolation,Identification and Drug Sensitivity Test of Clostridium perfringens from Broiler Chickens

为调查湖北地区健康鸡群中产气荚膜梭菌的分布状况,从规模化养鸡场采集鸡肛门棉拭子样品,应用厌气肉肝汤和CW琼脂培养基培养、紫牛乳反应、革兰染色及生化试验进行细菌的分离鉴定,应用多重PCR方法对分离菌株进行基因分型,并对分离菌株进行体外药敏试验。结果从120份样品中分离到9株(7.5%)产气荚膜梭菌,多重PCR都只扩增出α条带,毒素基因分型结果均为A型。药敏试验结果显示,分离菌株对链霉素、新霉素、丙氟哌酸、环丙沙星耐药,对丁胺卡那霉素,氯洁霉素中度敏感,对氨苄西林、阿莫西林、四环素、头孢噻肟高度敏感。

For investigating the prevalence and genetypes of C. perfringens in healthy chickens in Hubei province, 120 anus cotton swab samples were collected from large-scale broiler chicken farms in Hubei. After culturing in anaerobic meat liver soup and CW agar culture-medium, 9 suspected C. perfringens were isolated and then identified with purple milk test, Gram stain test and biochemical test. The isolation rate was 7.5 %. 9 strains were genotyped as type A by PCR. These strains were tested for susceptibility to common antimicrobials. All of the isolates were resistant to streptomycin, neomycin, ciprofloracin, ciprofloxacin, and were sensitive to ampicillin, amoxicillin, cefotaxime, tetracycline, amikacin and clindamycin.