陕西某奶牛场隐性乳房炎病原菌分离鉴定及药敏试验

Isolation and Identification of Subclinical Mastitis Pathogens and Drug Sensitivity Tests in A Dairy Cow Farm of Shanxi Province

为了解某场奶牛隐性乳房炎发生情况,应用LMT法对该奶牛场的165头临床健康奶牛进行隐性乳房炎检测,采集阳性乳样48份进行病原菌的分离鉴定,并对其主要病原菌进行了药敏试验。结果表明,该奶牛场奶牛隐性乳房炎阳性率为43.64%,乳区阳性率为24.46%,每个乳区均可发病,奶牛隐性乳房炎发病率与胎次和年龄有密切的关系,随着胎次和年龄的增加,奶牛隐性乳房炎发病率呈上升趋势;从阳性乳样中分离出11种107株细菌,主要病原菌为金黄色葡萄球菌、链球菌和大肠埃希菌。其中,金黄色葡萄球菌32株,占29.91%;链球菌15株,占14.02%;大肠埃希菌14株,占13.08%。3种主要病原菌对氧氟沙星、阿米卡星、环丙沙星敏感,对链霉素、青霉素、阿莫西林、氨苄西林耐药。说明可选择氧氟沙星、阿米卡星、环丙沙星作为治疗该奶牛场乳房炎的候选药物。

To understand the incidence of a recessive mastitis of dairy cows,using LMT to detect subclinical mastitis on 165 clinical healthy cows,48 milk samples with subclinical mastitis were detected,and the pathogens isolated,biochemical identified,and drug sensitivities were tested.The results showed that positeve rate of subclinical mastitis is 43.64%,the positive rate of udder quarter is 24.46%,all of mam-mary areas could catch the disease,the incidence of dairy subclinical mastitis closely connected with parity and age.One handred and seven strains bacteria were isolated,the main pathogenic bacteria are Staphylo-coccus aureus ,Streptococcus and E.coli.Among the 107 isolated pathogens,there were 32 strains of Staphylococcus aureus （29.91%）,15 strains of Streptococcus （14.02%）,14 strains of Escherichia coli （13.08%）.Three main pathogens were sensitive to ofloxacin,amikacin,ciprofloxacin,and resistant to streptomycin,penicillin,amoxicillin and Ampicillin.Dairy subclinical mastitis was on the rise with the in-crease of parity and age .Ofloxacin,amikacin,ciprofloxacin could be selected as the candidate drugs for treating the dairy subclinical mastitis in the farm.