柱花草胶孢炭疽菌致病力鉴定和致病类型分析

Pathogenicity Identification of Stylo Anthracnose Pathogens and Analysis of Pathogenic Types

用2套鉴别寄主分别对47份A型菌和57份B型菌的致病力进行鉴定,研究表明,不同的柱花草炭疽病菌株致病力不同。应用最大似然法(EML法)对鉴别寄主的致病力鉴定结果进行聚类分析,A型菌可划分成3大小种类型,且其聚类非常紧凑,表现出一定的寄生专化性;而B型菌可划分成2大小种类型,但其在型内分化较为复杂,说明B型菌将来有可能分化出更多的小种类型。从地区而言,无论A型菌还是B型菌均表现为海南菌株的致病力最强,其中尤以昌江和东方的菌株致病力最强。因而对病原变异和进化动态的监测十分必要。

104 isolates （47 with biotype A,57 with biotype B） were selected for race typing based on virulence and pathogenic variation determination by using a set of host differentials. The results showed different virulence in different isolates. Total 47 of type A isolates were grouped in 3 races, and the cluster is very compact. The hosts of type A isolates were S. scabra and S. hamata in this research. And total 57 of type B in 2 races. But the differentiation within the type is relatively complex, explain the type B could differentiate into more pathogenic types in the future. As for the region, regardless of the type A or type B strains were strongest for Hainan strain pathogenicity, notably the Changjiang and Dongfang strains pathogenic strength strongest. Thus more attention should be paid to the monitoring and evaluation.