银杏叶总黄酮的微波提取及纯化工艺研究

Study on microwave extraction and purification of total flavonoids from ginkgo leaf

通过正交设计确定银杏叶总黄酮用微波提取的最佳工艺,对溶剂浓度、料液比、提取时间和提取次数4个因素进行考察,确定其对银杏叶总黄酮提取率的影响,最佳提取条件为乙醇浓度60%、料液比1∶70、提取2次、提取时间3 min,银杏叶总黄酮得率为2.061%。银杏叶总黄酮进一步采用大孔树脂分离纯化,以黄酮含量为指标确定其最佳工艺参数,选定D101型大孔树脂,以80%乙醇、2倍柱体积/h的流速洗脱、收集乙醇洗脱液至3倍柱体积,为最佳工艺。经D101型大孔树脂纯化后,提取物中黄酮含量由17.08%提高到44.17%。

The optimum process of total flavonoids in Ginkgo biloba leaves by microwave extraction were determined by orthogonal design,the solvent concentration,solid-liquid ratio, extraction time and extraction times were studied to determine the effects on the extracting of total flavonoids,the optimum extraction conditions were ethanol concentration 60%,ratio of solid to liquid 1：70, extracting 2 times,3min every time,the rate was 2.061%. The total flavonoids in Ginkgo biloba leaves was further separated and purified by macroporous resin.The optimal conditions of resin purification were that using D101 type macroporous resin,80% ethanol as solvent,flow rate of elution was 2 times of column volume pre hour,and collecting alcohol eluent to 3 times of the column volume.After purification by resin,the content of total flavonoid was increased from 17.08% to 44.17%.