鲮鱼鱼皮胶原蛋白酶解物分子量分布与抗氧化性的研究(英文)

Analysis of Molecular Weight Distribution and Antioxidant Activities of Cirrhinus molitorella Skin Collagen Hydrolysates

[目的]为了研究鲮鱼鱼皮胶原蛋白水解物的分子量的分布及抗氧化活性。[方法]分子量分布采用分子排阻色谱法和基质辅助激光解吸附飞行时间质谱（MALDI-TOF-MS），鱼皮用碱性蛋白酶2709处理。[结果]最佳水解条件为：pH 10.0、反应温度55℃、底物浓度为80 g/L、酶和底物比4%，水解时间3 h。两种方法分析水解产物的相对分子质量分布范围为400-1800 Da,大多数胶原蛋白短肽的分子量在1400 Da以下。[结论]结果表明,鱼皮水解物是一种潜在的抗氧化物。

[Objective] The aim of this work was to identify molecular weight （MW） distribution and antioxidant activity of fish skin col agen hydrolysates. [Method] The MW distribution of hydrolysates was determined using both size exclusion chromatography and matrix-assisted laser desorption ionization time-of-flight mass spec-trometry （MALDI-TOF-MS）. Fish skin were treated by the alkaline protease 2709. [Result] The optional conditions for hyerolysis were time 3 h, temperature 55 ℃, pH 10.0, substrate concentration 80 g/L and E/S 4%. The results of both methods indi-cated that the molecular weight of col agen hydrolysates was from 400 to 1 800 Da, and the peptides’ molecular weight was less than 1 400 Da mostly. The reducing power and antioxidant/radical scavenging activity [1, 1-diphenyl-2-picryl-hydrazyl （DPPH） free radical scavenging activity] were determined. [Conclusion] The results reveal that the fishskin hydrolysate is a potential source of antioxidants.