湛江南药场引种檀香rDNAITS序列系统发育分析

Phylogenetic analysis of Santalum album in Zhanjiang based on rDNA ITS sequence

目的研究广东湛江南药场引种檀香与檀香科内植物rDNA ITS序列系统发育的关系。方法采用改良CTAB法提取檀香总DNA,分别对样品rDNA ITS区进行PCR扩增和测序,通过rDNA ITS序列进行分类鉴定及系统发育分析。结果 11个样品rDNA ITS序列间的遗传距离为0.000%-0.144%,邻接法和最大简约法构建的系统发育树显示国内引种自印度和印度尼西亚的檀香与印度檀香聚为一亚支（支持率为84%和95%）。结论基于rDNA ITS序列的测序分析和构建系统发育树的分子生物学方法,可为檀香药材的种类鉴定提供科学依据。

Objective To study on the phylogenetic relationship of the rDNA ITS sequences of Santalum album L. in Zhanjiang. Methods Total DNA was extracted with modified CTAB method and thereby the internal transcribed spacer （ITS） regions were amplified with universal primer and sequenced. Nueleotide sequences of rDNA ITS of Santalaceae were used for identification and phylogenetic analysis. Results Genetic distance of 11 sample sequences ranges from 0. 000% to 0. 144%. Phylogenetic trees conducted individually by maximum parsimony （MP） and neighbor-joining （NJ） of rDNA ITS indicated that Santalum album L. and sandalwood introduced from India and Indonesia formed a monophyletie clade. Conclusion Based on rDNA ITS region, molecular identification is useful in accurate classification on Santalaceae which provides molecular evidence of taxonomy and identification of different species in Santalaceae.