抗乙酰肝素酶单抗联合紫杉醇对肺癌A549增殖及侵袭的影响

Effect of anti-heparanase antibody combined with paclitaxel on the proliferation and invasiveness of A_(549) cells

目的探讨抗乙酰肝素酶(heparanase,HPA)单抗联合紫杉醇对肺癌A549细胞的增殖及侵袭抑制作用。方法选用浓度梯度递增的抗HPA单抗和紫杉醇,单独或联合作用于A549细胞48 h后,采用MTT法检测细胞增殖,流式细胞仪检测细胞周期,细胞划痕试验、Transwell试验检测细胞侵袭,观察抗HPA单抗与紫杉醇对细胞增殖及转移的抑制作用。结果抗HPA单抗与紫杉醇联用后将抑制率从20%、31%、37%、45%、51%增大至52%、56%、60%、67%、77%。两药分别干预后细胞S期分别降低至4.7%、6.8%,联用后出现了36.5%的细胞凋亡期。在体外侵袭实验中抗HPA单抗、紫杉醇、紫杉醇联用抗HPA单抗使肺癌A549细胞侵袭能力分别下降(23.3±5.3)%、(63.3±3.9)%、(85.0±3.2)%。结论抗HPA单抗联合紫杉醇对肺癌A549细胞的增殖及侵袭能力的抑制作用比两药单用时更为显著。

Objective To investigate the effect of anti-heparanase antibody combined with paclitaxel on the proliferation and invasiveness of A549 cells.Methods Anti-HPA antibody and paclitaxel were used alone or in combination to treat A549 cells with increased dosages.After 48 hours,MTT was used to detect the cell proliferation,flow cytometry was applied to measure the cell cycle,and cell scratch test and Transwell were adopted to observe the cell invasiveness.Results The inhibition rate of paclitaxel combined with anti-HPA antibody increased from 20%,31%,37%,45% and 51% to 52%,56%,60%,67% and 77%,respectively, in a dosage-dependent manner.The S phase of cell cycle was reduced to 4.7% and 6.8% when A549 cells treated with paclitaxel and anti-HPA antibody,respectively.However,paclitaxel combined with anti-HPA antibody led to 36.5% apoptosis.In addition,the invasiveness of A549 cells decreased（23.3±5.3） %、（63.3 ±3.9） % and（85.0±3.2） % when treated with anti-HPA antibody,paclitaxel and paclitaxel combined with anti-HPA antibody,respectively.Conclusion Combination of anti-HPA antibody and paclitaxel for inhibition of A549 cells proliferation and invasiveness is more significant than only one drug used.