菌种筛选对1型肺炎链球菌产糖量的影响

Effect of bacterial colony screening on polysaccharide yield of Streptococcus pneumoniae serotype 1

目的用无动物源性培养基筛选高产糖1型肺炎链球菌（Streptococcus pneumoniae semtype1，PN1）。方法配制无动物源性培养基，并用此培养基进行高产糖PN1单菌落筛选。比较筛选前后PN1在发酵培养中的产糖量。结果无动物源性培养基筛选前后PN1在发酵培养中的产糖量分别为420和960mg／L，筛选后PN1的产糖量明显高于筛选前PN1。结论以无动物源性培养基筛选PNI可使PN1的产糖量明显提高。

Objective To screen a colony of Streptococcus pneumoniae serotype 1 （PN1） with high polysaccharide yield using animal-free medium. Methods The animal-free medium was prepared and used to screen a single colony of PN1 with high polysaceharide yield. The polysaecharide yield of PN1 in fermentation culture was compared between before and after screening. Results The polysaccharide yields of PN1 before and after screening with the animal-free medium were 420 and 960 mg/L in fermen- tation culture, respectively. The polysaccharide yield of PN1 was significantly higher after screening than before screening. Conclusion PN1 screening with animal-free medium can significantly increase polysaccharide yield of PN1.