无细胞百日咳疫苗特异性毒性检测方法现状及展望

Current status and prospect of specific methods for determination of toxicity of an aceilular pertussis vaccine

百日咳毒素是百日咳杆菌重要的保护性抗原，经化学方法脱毒成为百日咳类毒素后制成的无细胞百日咳疫苗（acellular pertussis vaccine，APV）具有残余毒性和毒性逆转的可能性，所以建立特异性毒性检测方法对于保证APV的安全性是十分必要的。此文就国内外APV特异性毒性检测方法的现状做一综述，并结合近几年国外研究的毒性检测新方法进行展望。

Pertussis toxin （PT） is a major protective antigen of Bordetella pertussis and its detoxified form obtained by a chemical process is an important component of an acellular pertussis vaccine （APV）. In view of the possibility of residual toxicity or toxicity reversion of PT in the vaccines, specific tests are required to assure safety of the vaccines. This paper reviews specific methods for determination of toxicity of APV and prospect of new tests in the world.