大肠埃希菌和肺炎克雷伯菌β-内酰胺酶的筛选

目的了解大肠埃希菌和肺炎克雷伯菌中β-内酰胺酶的分布、表型及其检测方法。方法应用纸片法进行初筛后,分别用美国临床实验室标准化委员会(National Committee for Clinical Laboratory Standards,NCCLS)推荐的确证试验检测超广谱β-内酰胺酶(Extended Spectrum BetaLactamases,ESBLs)和用头孢西丁三相试验检测AmpCs酶。结果 367株大肠埃希菌和肺炎克雷伯菌中经ESBLs药敏初筛和确证试验,有115株(31.3%)产ESBL,有17株(4.6%)头孢西丁三相试验阳性者。结论本院大肠埃希菌和肺炎克雷伯菌中产β-内酰胺酶以ESBLs为主,AmpC酶占4.6%。

Objective To study distribution,phenotypes and detection methods ofβ-lactamases in Escherichia coli and Klebsiella pneumoniae. Methods Using Kirby-Bauer agar diffusion test for screening,extended spectrumβ-lactamases （ESBL） confirm test which National Committee for Clinical Laboratory Standards（NCCLS） recommended for detecting ESBLs,and cefoxitin three-dimension test for detecting AmpCsβ-lactamases. Results Among 367 clinical isolates of Escherichia coli and Klebsiella pneumoniae,there are 115 strains （31.3%） showed production of ESBLs by screening and confirming tests,and 17 stains（4.6%） showed production of AmpCβ-lactamases by cefoxitin three-dimensional tests. Conclusion ESBLs （31.3%） were the mainβ-lactamases in Escherichia coli and Klebsiella pneumoniae in the hospital and the detection rate of AmpCβ-lactamases was 4.6%.