维药迪娜口服液对急性肝损伤小鼠肝脏组织MDA、GSH-Px含量及Ca^(2+)-ATPase活性的影响

Effects of Uyghur Medicine Dina oral liquid on acute liver injury in mice liver tissue MDA,GSH-Px content and Ca^(2+)-ATPase activity

目的探讨维药迪娜口服液对四氯化碳(CCl4)致急性肝损伤小鼠肝组织丙二醛(MDA)含量、谷胱甘肽过氧化物酶(GSH-Px)活力及Ca2+转运ATP酶(Ca2+-ATPase)活性的影响及其保肝作用机制。方法取小鼠60只随机分成正常对照组、模型组、阳性对照药联苯双酯组(3.75mg/kg)及迪娜口服液低、中、高剂量组(分别为4.78、9.55、19.10g迪娜口服液/kg体质量,相当于人用量95.5g/d的5、10、20倍),每组10只。各实验药物组及阳性对照组分别按剂量灌胃给药0.2mL/10g体质量,正常对照组、模型组给予等容量蒸馏水;连续给药7d,禁食不禁水12h,除正常对照组外,其他各组动物腹腔注射0.15%CCl4-花生油溶液(0.1mL/10g)。造模24h后取肝脏做组织匀浆,检测MDA含量、GSH-Px活力与Ca2+-ATPase活性的变化。结果与正常对照组比较,模型组MDA含量明显升高,GSH-Px活力与Ca2+-ATPase活性均明显下降,差异有统计学意义(P<0.05);与模型组比较,阳性对照组和迪娜各剂量组MDA含量均降低,GSH-Px活力及Ca2+-ATPase活性提高,其中迪娜中剂量组MDA含量降低及组织Ca2+-ATPase活性提高最为明显(P<0.05),迪娜高剂量组GSH-Px活力上升较为明显。结论迪娜口服液对CCl4所致小鼠急性肝损伤具有明显的保护作用,其机制可能与清除自由基损伤和保护酶活性有关。

Objective To sludy the protective effect of Uyghur Medicine Dina oral liquid on CCl4-induced acute liver injury in mice. Methods The mice were randomly divided into normal control group and CCl4- induced acute liver injury model group, bifendate treatment group （positive control, at 3.75 mg/kg） and Dina oral liquid treatment group （small dose group, at 4.78 g/kg; middle dose group, at 9.55 g/kg and high dose group,at 19.1 g/kg）. The administration of bifendate and Dina oral liquid was performed once a day for 7 days. The model, bifendate treatment and Dina oral liquid treatment groups were intragastrically given 1.5% CCl4 in salad oil solution at the dosage of 1 mL/kg to induce acute liver injury, and normal control group intraperitoneal injection of the same dose of vegetable oil. At 24 hour after the administration of CCl4, all the mice were sacrificed to assay antioxidant activities in the blood. Meanwhile, hepatic histopathological changes were also examined. Results The MDA level in the liver and serum of acute hepatic injury model group were significant higher than those in normal control group, while the content of the GSH-Px and Ca^2＋-ATPase were significantly lower than that in model control group, compared with the acute hepatic injury model group, the MDA levels in the liver in Dina oral liquid all groups were both evidently decreased, especially in middle dose group; while the content of GSH-Px and Ca^2＋-ATPase were significantly higher than that in model control group, particularly in Dina oral liquid middle dose group （Ca^2＋-ATPase） and in high dose group （GSH-Px）. Conclusion Dina oral liquid have protective effects on acute hepatic injury induced by CCl4 in mice.