HPLC法测定牛至中迷迭香酸的含量

Determination of Rosmarinic acid content in Origanum vulgare L.by HPLC

目的 建立牛至中迷迭香酸含量的HPLC测定方法 。方法 采用高效液相色谱法（HPLC）测定牛至中迷迭香酸的含量。色谱条件：Kromasil C18柱（4．6mm×150mm，5μm），流动相为甲醇-1．5％乙酸水（38：62），流速为1．0mL／min，检测波长为330nm，柱温25℃。结果 迷迭香酸峰面积与进样量在0．3321～1．6605μg范围内有良好的线性关系（r=0．9999），平均回收率为98．96％，RSD为1．09％（n=6）。10个产地的牛至中迷迭香酸含量为8．13-15．74mg／g。结论 HPLC法操作简单、准确可靠，可用于牛至中迷迭香酸含量的测定。

Objective To establish a method for the content determination of Rosmarinic acid in Origanum vulgareL. Methods The content of Rosmarinic acid was detected by HPLC. The separation was carried out on a Kromasil C18 column （150 mm×4.6 mm, 5 μm）. The mobile phase was Methanol -1.5% Acetic acid solution （38 ： 62）; the flow rate was 1.0 mL/min; the detective wavelength was set at 330 nm. The column temperature was 25 ℃. Results The calibration curve showed good linearity over the range of 0.332 1-1.660 5 μg （r =0.999 9）. The average recovery was 98.96% with RSD 1.09% （n =6）. The content of Rosmarinic acid in Origanurn vulgare L. from 10 different regions were between to 8.13-15.74 mg/g. Conclusion This method was rapid, accurate, reliable; it could be used as a determination of Rosmarinic acid in Origanurn vulgare L.