尼可地尔对阿霉素损伤大鼠心肌的保护作用

Protection effects of Nicorandil on myocardial of rats dameged by Adriamycin

目的探讨尼可地尔对阿霉素损伤大鼠心肌的保护作用。方法将45只雄性SD大鼠用随机数字表法分为对照组(A组,n=15)、阿霉素模型组(B组,n=15)、尼可地尔+阿霉素组(C组,n=15)。B、C组大鼠每周经腹腔注射阿霉素2.5 mg/kg,A组大鼠每周经腹腔注射生理盐水2.5 mg/kg,共持续6周。第8周开始C组大鼠每次灌胃法灌入尼可地尔3 mg/kg,1次/d,持续30 d。之后通过心脏彩超检测三组大鼠的心脏功能并将其处死。用天狼星红染色法检测各组大鼠心肌的胶原容积分数。结果①造模结束时,B组大鼠平均体重小于A、C组(P<0.01或P<0.05)。②超声结果显示:与A组比较,B组大鼠左室收缩末内径(LVEDS)、收缩末期容积(ESV)明显增大(P<0.01),左室射血分数(LVEF)、左室短轴收缩率(FS%)明显降低(P<0.01);与B组比较,C组LVEDS、ESV明显减小(P<0.01),LVEF、FS%明显增高(P<0.01)。③病理结果显示:B组心肌组织胶原容积分数明显比A组高(P<0.01),而B、C两组比较差异无统计学意义(P>0.05)。结论尼可地尔对阿霉素损伤心肌有保护作用。

Objective To investigate the protection effect of Nicorandil on myocardial of rats dameged by Adriamyein. Methods 45 male SD rats were randomly divided into control group （group A, n=15）, Adriamycin group （group B, n= 15） and Adriamyein combined with Nieorandil group （group C, n=15）. The rats in group B and C were performed with intraperitoneal injection of Adriamyein at the dose of 2.5 mg/kg a week , those in group A were performed with in- traperitoneal injection of normal saline at the dose of 2.5 mg/kg a week, for 6 weeks. From the eighth week, rats in group C were performed with Nieorandil by stomach lavaging at the dose of 3 mg/kg once a day for 30 days. Then the rats were sacrificed after cardiac function accessed by echoeardiography. Meanwhile, collagen volume fraction was mea- sured in each group by sirius red staining. Results ①At the end of molding, mean weight of rats in group B was lighter than those in group A and C （P 〈 0.01 or P 〈 0.05）. ②Echocardiography showed that LVEDS and ESV in group B were obviously larger than those in group A and C （P 〈 0.01）, whlie LVEF and FS% were obviously lower than those in group A and C （P 〈 0.01）. ③The results of pathology showed that the collagen volume fraction in group B was higher than that in group A （P 〈 0.01）, and there was no statistical difference between group B and group C in collagen vol- ume fraction （P 〉 0.05）. Conclusion Nieorandil has protection effects on myocardial of rats dameged by Adriamyein.