气相色谱法测定新疆昆仑雪菊挥发油中柠檬烯的含量

Determination of limonene in volatile oil from Xinjiang Kunlun Chrys-anthemum by GC

目的建立新疆昆仑雪菊中柠檬烯含量的测定方法。方法采用气相色谱法，Rtx-5石英毛细管柱（30m×0．25mm，0．25μm）；程序升温：50℃保持5min，以10℃／min升至230℃，保持10min；载气流量：18．30mL／min；柱压：100kPa；进样口温度：250℃；分流比：10：1；进样量：1μL。结果柠檬烯在41.90～251．40μg／mL范围内线性关系良好（r=0．9996，n=6），平均回收率为102．72％，RSD为1．35％（n=9）。结论本实验方法可靠，重复性好，专属性强．可用于雪菊挥发油中柠檬烯的含量测定，以控制雪菊挥发油的质量。

Objective To establish a method for determination of limonene in Xinjiang Kunlun Chrysanthemum by GC. Methods The GC equipped with FID detector, and a Rtx-5 capillary column were used （30m×0.25 mm, 0.25 μm）. The temperature program of column oven was set as follows： 50℃ for 5 min, increased to 230℃ at a rate of 10℃/min, held for 10 min. The carrier gas flow rate： 18.30 mL/min; column press： 100 kPa; injection port temperature： 250℃; split ration： 10：1; sample volume： 1μL. Results Limonene was linear in the range of 41.90-251.40 μg/mL （r = 0.9996, n = 6）. The average recovery was 102.72% （n = 9）, and RSD was 1.35%. Conclusion The method can be used to determine the con- tent of limonene in Xinjiang Kunlun Chrysanthemum, in order to control the quality of volatile oil from Chrysanthemum.