培养法检测支原体肺炎假阳性存在因素及临床价值

The factor and clinical value of culture method in testing false positive of mycoplasma pneumonia

目的：探讨在培养法中影响支原体肺炎产生假阳性的因素以及其临床意义．方法：以在我院门诊治疗的360例肺炎患者为研究对象，采集其痰标本并进行培养，测得肺炎支原体的阳性率．再对筛选出来的阳性标本继续进行培养，并在培养基中加入抑制念珠菌生长的氟康唑，再次测得肺炎支原体阳性率．结果：在360例患者标本中，肺炎支原体阳性120例，其阳性率为33．3％；在加入氟康唑的120例培养标本中，肺炎支原体阳性76例，其阳性率为63．3％．两组标本中肺炎支原体阳性率存在显著性差异（P〈0．05）．结论：在呼吸道感染的诊断中，传统方法对其培养会存在一定的假阳性，应进一步进行培养，排除杂菌念球菌的对培养标本的影响．为临床上尽快诊断支原体肺炎提供可靠依据，具有重要临床价值．

AIM： To investigate the effects of mycoplasma pneumonia false positive and its clinical significance in the culture method. METHODS： We took 360 cases of pneumonia patients treated in our clinic for the study, and collected and cultured their sputum samples. Then we measured positive rate of mycoplasma pneumonia. Then we screen out again to continue culture positive specimens, and added fluconazole to the medium that could inhibit the growth of Candida, and measured again the positive rate of mycoplasma pneumonia. RESULTS： In 360 patients samples, there were 120 cases of mycoplasma pneumonia positive samples, the positive rate of 33.3% ; After joining fluconazole in 120 patients in cultured specimens, there were 76 cases of mycoplasma pneumonia positive, the positive rate was 63.3%. There were significant differences between the two groups specimens of myco- plasma pneumonia positive rate（P 〈0.05）. CONCLUSION： In the diagnosis of respiratory infections, the traditional methods for their training will be some false positives. So we should further develop, excluding the impact of bacteria on culture specimens such as Candida. As soon as the clinical diagnosis of mycoplasma pneumonia, which could provide a reliable basis that has important clinical value.