野百合碱诱导的肺动脉高压大鼠TGF-β1/Smad2信号通路表达的研究

The expression of TGF-β1 /Smad2 signaling pathway in monocrotaline-induced pulmonary hypertension in rats

目的:研究TGF-β1/Smad2信号通路在野百合碱诱导的肺动脉高压大鼠中的表达。方法:18只雄性SD大鼠随机分为对照组和模型组,采用皮下注射野百合碱(60 mg/kg)的方法复制肺动脉高压模型。野百合碱注射5w后,测定大鼠右心室收缩压(RVSP)和右心室肥厚指数(RVHI)。Western blot检测肺组织中TGF-β1、p-Smad2和Smad2的表达水平。结果:模型组大鼠的RVSP、RVHI明显高于对照组,模型组大鼠肺组织中TGF-β1、p-Smad2的表达明显高于对照组。结论:TGF-β1/Smad2信号通路可能参与了肺动脉高压发病过程。

Objective：To explore the expression of TGF-IM/Smad2 signaling pathway in monocrotaline induced pulmonary hypertension in rats. Methods： 18 male Sprague-Dawley rats were randomly divided into control group and model group. The rats in the model group were underwent a subcutaneous injection of 60 mg/ kg monocrotaline（MCT）. Five weeks after injection, fight ventricular systolic pressure （RVSP） and right ventficular hypertrophy index （RVHI） were measured. The expression levels of TGF-β1, p-Smad2 and Smad2 in the lung tissue were detected by Western blot. Results： RVSP and RVHI in the model group were significantly higher than that in the control group. The expression of TGF-β1 and p-Smad2 in the lung was also significantly higher than that in the control group. Conclusion： TGF-β1/Smad2 signaling pathway may be involved in the pathogenesis of pulmonary hypertension.