鱼腥草及三丫苦对甲型流感病毒NPAG表达的影响

Effect of expression of Yuxincao and Sanyaku on influenza a virus nucleoprotein antigen

目的探讨鱼腥草及三丫苦对甲型流感病毒(nucleo protein antigen,NPAG)表达的影响,了解鱼腥草及三丫苦抗甲型流感病毒的分子机制。方法以胶体金法测定NPAG的表达。瞬间转染,实验分为:Hela细胞组、空质粒组[PCDNA3.1(+)/empty]、重组质粒组[PCDNA3.1(+)/NP)]、脂质体组、三丫苦醇提物组和鱼腥草油组。药物实验加入重组质粒同时将药物加入培养细胞孔中进行干预。转染48h后,测定其上清液NPAG的表达。结果胶体金法测试显示:Hela细胞组、PCDNA3.1(+)/empty组、脂质体组、鱼腥草油组为阴性;PCDNA3.1(+)/NP组、三丫苦醇提物为阳性,NPAG浓度1:64。结论鱼腥草油组可抑制甲型流感病毒NPAG的表达,能抗甲型流感病毒。三丫苦醇提物组不影响NPAG的表达。

Objective To investigated the effect of Yuxincao and Sanyaku on expression of influenza a virus nucleoprotein antigen （NPAG）,Mechanism of Yuxincao and Sanyaku ant influenza a virus on the molecular level. Methods Colloidal gold im-munochromatography methods using to measure NPAG expression of Hela cell group,PCDNA3.1（＋）/empty group,PCDNA3.1（＋）/NP group, liposome group,Sanyaku group and Yuxincao group. the successfully transfected HeLa cells as target cells. 48 hours after transfection,the supernatant was used to test the expression of NPAG. Results Collodial gold showed Hela cell group and PCD-NA3.1（＋）/empty group were negative,PCDNA3.1（＋）/NP group and Sanyaku group were NPAG positive. Titration of NPAG were 1：64. Conclusion Yuxincao group could inhibit NPAG expression of influnte A Virus,Sanyaku group not could inhibit.