海洋来源真菌的抗肿瘤抗真菌活性筛选

Antitumor and antifungal activities screening of deep sea sediment derived fungi

目的:将深海来源的16株真菌经发酵培养与活性筛选,获取活性菌株以供筛选药源活性产物。分别经真菌普通培养基和人工海水培养基发酵获得样品,采用MTT法测试抗肿瘤活性,纸片法测试抗真菌活性。结果:菌株中有3株经过普通发酵培养基发酵和4株经过海水培养基发酵的样品在100μg/mL浓度下对K562细胞的抑制率大于60%;抗真菌活性测试中,仅有菌株16-02-1的发酵样品对受试白色念珠菌ATCC 10231和土曲霉W-1均呈现一定的抑制活性。结论:深海来源真菌在不同培养基中发酵获得的样品,抗肿瘤活性各不相同,经过筛选获得高活性菌株为寻找药源活性产物提供了菌株。

Abstrat： Objective：To investigate metabolites of sixteen strains isolated from a deep sea sediment for obtaining the bioactive metabolite producing strains. Methods：Sixteen fungi were fermented with ordinary medium and artificial seawater medium. Antitumor and antifungal activities were tested by MTT and paper-disc methods, respectively. Results There are 3 fungal strains fermented by ordinary medium among them inhibited the proliferation of K562 cells with the inhibition rates over 50% at the 100 μg/mL of concentration, samples from 5 fungal strains fermented by artificial seawater inhibited the proliferation of K562 cells with the inhibition rates over 40% at the 100 μg/mL of concentration, only Strain 16-02-1 revealed both antitumor and antifungal activities. Conclusions：The results indicated that the production have different inhibitive femented in different medium, and obtain strains with high activity source for finding the active drug product provides strain.