摘要
目的探讨亚甲基四氢叶酸还原酶(MTHFR)基因667位点多态性在青岛市汉族育龄妇女中的分布状况。方法用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测98份育龄妇女外周血MTHFR C667T基因型分布。结果本研究人群MTHFR 677位点基因的CC、CT、TT基因型频率分别为24.5%、53.1%、22.4%,T等位基因频率为0.49。结论青岛市汉族育龄妇女MTHFR基因667位点多态性与其他地区有较大不同,调查可以为相关研究提供相应的分子生物学依据。
Objective: To investigate the distribution of C667T polymorphisms of methylene tetrahydrofolate reductase (MTHFR) gene among Han women childbearing age in Qingdao. Methods: DNA MTHFR genotype was detected with PCR- RFLP in 98 blood samples of the normal Hun women of childbearing age. Rseults= The frquencies of the C667T genotypes were as follows: CC. 24.5%; CT: 53.1%;TT: 22.4%.The allele frequencies of 677T was 0.49. Conclusion: In Hun women childbearing age in Qingdao, MTHFR 677 polymorphisms is different form those reported previously.Studies can povide molecular biological foundation for relate research.
出处
《中国优生与遗传杂志》
2014年第6期6-7,共2页
Chinese Journal of Birth Health & Heredity
关键词
亚甲基四氢叶酸还原酶
基因型
汉族育龄妇女
青岛市
Methylene tetrahydrofolate reductase
Genotype
Han women of childbearing age
Qingdao