青岛地区汉族育龄妇女亚甲基四氢叶酸还原酶基因667位点多态性分布研究

Genetic polymorphism of methylene tetrahydrofolate reductase c667t in han women of childbearing age in Qingdao

目的探讨亚甲基四氢叶酸还原酶(MTHFR)基因667位点多态性在青岛市汉族育龄妇女中的分布状况。方法用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测98份育龄妇女外周血MTHFR C667T基因型分布。结果本研究人群MTHFR 677位点基因的CC、CT、TT基因型频率分别为24.5%、53.1%、22.4%,T等位基因频率为0.49。结论青岛市汉族育龄妇女MTHFR基因667位点多态性与其他地区有较大不同,调查可以为相关研究提供相应的分子生物学依据。

Objective： To investigate the distribution of C667T polymorphisms of methylene tetrahydrofolate reductase （MTHFR） gene among Han women childbearing age in Qingdao. Methods： DNA MTHFR genotype was detected with PCR- RFLP in 98 blood samples of the normal Hun women of childbearing age. Rseults= The frquencies of the C667T genotypes were as follows： CC. 24.5%; CT： 53.1%;TT： 22.4%.The allele frequencies of 677T was 0.49. Conclusion： In Hun women childbearing age in Qingdao, MTHFR 677 polymorphisms is different form those reported previously.Studies can povide molecular biological foundation for relate research.