树突状细胞C型凝集素在自然流产中的表达研究

The expression of DC-SIGN on dendriticcells in spontaneous abortion

目的通过检测早期自然流产患者子宫蜕膜组织中树突状细胞C型凝集素(DC-SIGN)的表达,探讨其在自然流产发生中的可能机制。方法采用免疫荧光染色法及实时荧光定量PCR(RT-PCR)技术检测DC-SIGN在蜕膜组织中的表达,比较两组间表达情况及分布差异。结果 1.通过免疫荧光染色技术检测显示:妊娠期子宫蜕膜组织均表达DC-SIGN,主要分布于细胞膜上,自然流产组中DC-SIGN+DC表达明显低于对照组(46.20±2.40 vs 78.21±0.28,P<0.01)。2.RT-PCR结果显示:自然流产组明显低于对照组,患者蜕膜组织中DC-SIGN mRNA相对表达量(0.0032370±0.00124 vs0.0076412±0.0040,P<0.05)。结论树突状细胞(DC)在母胎免疫耐受机制中起着重要的作用,蜕膜组织DC-SIGN的表达量降低可能导致早期自然流产的发生。

Objective：To investigate the expression of DC-SIGN on the dendritic cells in spontaneous abortion and the possible role in the mechanism of spontaneous abortion. Methods：Immunofluorescence and RT-PCR to detect DC-SIGN expression in the decidual tissue. Compared with group of spontaneous abortion and family planning group expression and distribution differences. Results：1.By immunofluorescent technique for detection of visible natural abortion group and control group with DC-SIGN expression,mainly distributed in the membrane of the cell. using ImageJ software to analyze the number of positive cells,respectively：46.20±2.40 vs 78.21±0.28,spontaneous abortion group in the DC-SIGN ＋ DCs express less than the control group,and there was significantly different（t = 63.03,P0.01）. 2.By using the techniques of RT-PCR test results show that：the group of spontaneous abortion decidua of normal DC-SIGN mRNA relative expression was significantly less than that in the control group,and there was statistical significance（P =0.02,P0.05）. Conclusions：Dendritic cells（DC）play an important role in the maternal-fetal immune tolerance mechanisms,and expression of DC-SIGN decrease in the occurrence of spontaneous abortion may play a certain role.