趋化因子MCP-1、MIP-1α的表达与TAM计数在胆囊癌中的临床病理意义

Clinicopathological Significance of Expression of Chemokine MCP-1,MIP-1α and TAM Count in Gallbladder Carcinoma

目的:检测胆囊腺癌组织中趋化因子MCP-1和MIP-1α的表达、TAM计数并探讨其临床病理意义。方法:收集中南大学湘雅二医院及湖南省人民医院近五年胆囊腺癌手术切除标本36例及慢性胆囊炎手术切除标本10例,采用原位分子杂交方法检测MCP-1和MIP-1α的表达,免疫组化法进行TAM计数。结果:胆囊腺癌组织中MCP-1、MIP-1αmRNA表达阳性率及评分均明显高于慢性胆囊炎(P<0.01);高分化胆囊腺癌中二者的阳性率及评分均低于低分化胆囊腺癌,其中MCP-1 mRNA比较有显著性差异(P<0.05);MCP-1、MIP-1αmRNA的表达呈显著正相关。胆囊腺癌组织MCP-1 mRNA表达阳性率及其评分与侵犯胆总管及发生淋巴结转移显著相关;MIP-1αmRNA表达阳性率及其评分与侵犯肝脏显著相关。胆囊腺癌组织中,TAM计数(24.89±0.84)明显高于慢性胆囊炎组织(16.19±0.66),有显著性差异(P<0.01)。TAM与MCP-1、MIP-1αmRNA表达评分值均呈显著正相关(r分别为0.580,0.567)。MCP-1mRNA与MIP-1αmRNA评分值之间呈显著正相关(r=0.638)。结论:MCP-1、MIP-1α的表达增加及TAM计数升高可能调控和影响胆囊癌的发生和发展,MCP-1、MIP-1α可能促进TAM向胆囊癌组织迁移浸润。

Objective： Expression ofMCP-1, MIP-1α and TAM count in gallbladder carcinoma tissue were detected in this study, and their clinical significance were analyzed. Methods： 36 cases of gallbladder carcinoma operation samples and 10 cases of chronic cholecystitis operation samples were collected from Hunan province People＇s Hospital and Second Xiangya Hospital. In situ hybridization method was used to detect the expression of MCP-1 and MIP-1 α, ABC immunohistochemical method was used for TAM count. Results： In gallbladder carcinoma, the positive rate and score of MCP-1 and MIP-1α mRNA expression was significantly higher than that of the chronic cholecystitis（P〈0.01）; The positive rate and score of high differentiation gallbladder adenocarcinoma were lower than those of the low differentiation gallbladder adenocarcinoma, while the MCP-lmRNA expressed significantly differently between different differentiation groups （P〈0.05）; significant positive correlation was found betwween MCP-1 and MIP-1 α mRNA expression. In gallbladder adenocarcinoma tissue, the positive rate and score ofMCP-1 mRNA expression were significantly related to the infringement of common bile duct and the incidence of lymph node metastasis; the positive rate and score of MIP-1 αrnRNA expression were significantly related to the invasion of the liver. In 36 cases of gallbladder carcinoma tissue, TAM count （24.89± 0.84） was significantly higher than that of 10 cases of chronic cholecystitis group （16.19± 0.66） with significant difference （P〈0.01）. TAM count of MCP-1 and MIP-1α mRNA positive cases were significantly higher than those in negative cases （P〈0.01）. TAM and MCP-1, MIP-1 α mRNA expression score showed significant positive correlation（r=0.580, 0.567）. Score of MCP-lmRNA and MIP-1α mRNA has a close positive correlation （r=0.638）. Conclusion： The increased expression of MCP-1, MIP-1 αand TAM count enhencement may regulate and influence the occurrence and development of gallbladder carcinoma, MCP-1, MIP-1 α may promote TAM to gallbladder carcinoma tissue infiltration.