一种复方二氧化氯消毒剂杀菌效果及其杀菌机理的研究

GERMICIDAL EFFICACY AND MECHANISM OF A COMPOUND CHLORINE DIOXIDE DISINFECTANT

目的研究一种复方二氧化氯消毒剂对大肠杆菌的杀灭效果及其杀菌机理。方法采用悬液定量杀菌试验和透射电镜的方法,对该复方二氧化氯消毒剂杀菌效果及其杀菌机理进行实验室观察。结果用含50 mg/L二氧化氯的该复方消毒剂对悬液内大肠杆菌作用10 min,平均杀灭率达到99.9%以上。透射电镜观察发现经二氧化氯作用后的大肠杆菌菌体超微结构受到明显破坏。蛋白质渗漏实验表明受二氧化氯作用后的大肠杆菌细胞内容物大量漏出。结论该复方二氧化氯消毒剂在较低浓度下可有效杀灭大肠杆菌,其主要作用机理是致使菌体超微结构破坏和细胞内容物漏出。

Objective To study the germicidal efficacy and mechanism of a compound chlorine dioxide disinfectant on Escherichia coli. Methods The suspension quantitative germicidal test and TEM method were used to study the germicidal efficacy and mechanism of the compound disinfectant. Results The average killing rates of Escherichia coli exposed to compound disinfectant solution containing 50 mg/L chlorine dioxide for 10 minutes were all above 99.9%. Observation of transmission electron microscopic showed that chlorine dioxide destroyed the ultrastructure of Escherichia coli cells obvious- ly. Protein leakage experiments showed that the cellular contents of Escherichia coli leaked largely after chlorine dioxide ex- posure. Conclusion The compound chlorine dioxide disinfectant can effectively kill Escherichia coli in low concentration, and the mechanism is to cause the damage of cell ultrastructure and the leakage of cellular contents.