固相萃取-高效液相色谱荧光法检测辣椒制品中的罗丹明B

Detection of Rhodamine B in hot pepper by fluorescence solid phase extraction-high performance liquid chromatography

建立一种固相萃取-高效液相色谱荧光法检测辣椒制品中罗丹明B的方法。试样经固相萃取小柱净化、浓缩，以乙腈-乙酸铵溶液为流动相，保留时间定性，外标法定量。罗丹明B在1．0～100．0μg·L^-1。范围内线性关系良好，相关系数大于0．9999，相对标准偏差在1．6％～4．3％之间，回收率在85．6％～102．8％之间。该方法灵敏度高，精密度和重现性好，可操作性强，具有较高的实际应用价值。

Solid phase extraction combined with SPE - HPLC fluorescence detector to determine Rhodamine B in pepper was established. The extracts were purified, concentrated by HPLC using acetonitrileammonium acetate solution as mo- bile phase. The retention time was used as qualitative analysis and external standard was used as quantified detecting. Rhodamine B at 1.0 - 100. 0 μg L - 1 showed a good linear relationship. The correlation coefficient was larger than 0. 9999, the relative standard deviation was 1.6% - 4. 3% , the recovery rate was 85.6% - 102. 8%. The method had high sensitivity, good precision and repeatability. It has high practical application value.