HPLC法测定竹叶提取物中荭草苷、异荭草素、牡荆素和异牡荆素的含量

Determination of Orientin,Isoorientin,Vitexin and Isovitexin in Bamboo Leaf Extract by HPLC

目的:建立竹叶提取物中荭草苷、异荭草素、牡荆素和异牡荆素含量的HPLC测定方法。方法:色谱柱:Agilent HC-C18(250mm×4.6mm,5μm);流动相:乙腈-2%冰醋酸(14∶86);流速:1mL/min;检测波长350nm;柱温:40℃。结果:线性范围为荭草苷在2.0760~12.4560μg(r=0.9999);异荭草素在3.9400~23.6400μg(r=0.9997);牡荆素在0.2740~1.6440μg(r=0.9999);异牡荆素在0.7690~4.6140μg(r=0.9999)。荭草苷平均回收率为99.36%,RSD为2.39%;异荭草素平均回收率为99.69%,RSD为1.89%;牡荆素平均回收率为100.04%,RSD为1.49%;异牡荆素平均回收率为100.64%,RSD为1.61%。结论:该方法准确可靠、简单易行,适用于竹叶提取物中荭草苷、异荭草素、牡荆素和异牡荆素的含量测定。

Objective：To establish the method of determination of Orientin , Isoorientin, Vitexin and Isovitexin in Bamboo leaf extract . Methods：The chromatographic conditions included Agilent HC -C18 column（250mm ＆#215;4.6mm, 5μm）and the mobile phase consisted of Acetonitrile -2%glacial acetic acid（14∶86）.Detection wavelength was at 350 nm.The flow rate was 1.0 mL/min.Column tempera-ture was at 40℃.Results：The linear range for Orientin was 2.0760-12.4560μg（r=0.9999）, those for Isoorientin was 3.9400μg^23.6400μg（r=0.9997）, those for Vitexin was 0.2740~1.6440μg（r=0.9999）, and those for Isovitexin was 0.7690~4.6140μg（r=0.9999）.The average recovery and RSD for Orientin were 99.36%and 2.39%, those for Isoorientin were 99.69%and 1.89%, those for Vitexin were 100.04%and 1.49%,and those for Isovitexin were 100.64%and 1.61%.Conclusion：The method is reliable and accurate ,simple and feasible ,it can be used for the content determination of Bamboo leaf extract .