摘要
[目的]探讨p53及MMP-2蛋白在人肺癌组织中的表达情况。[方法]采用量子点免疫荧光组织化学(QDs-IHC)技术分析和研究人肺癌组织芯片中p53及MMP-2蛋白的表达情况,并检测p53及MMP-2蛋白在肺癌组织中的共表达。[结果]QDs-IHC检测分析显示,p53及MMP-2蛋白在肺癌组的表达均高于对照组,差异显著(P<0.05)。p53及MMP-2蛋白表达之间呈显著正相关(r=0.533,P<0.01)。[结论]新型半导体免疫荧光标记试剂—量子点结合组织芯片技术(QDs-IHC)可以检测到p53及MMP-2蛋白在肺癌组织中的表达,p53及MMP-2在肺癌组织中的共表达对肺癌的恶性进展起促进作用。
[Purpose ] To investigate the expression of p53 and MMP-2 in lung carcinoma tissues. [Methods ] Expression of p53 and MMP-2 in lung carcinoma tissues and normal lung tissues was detected by quantum dots combined with IHC technology (QDs-IHC). [Results] Expressions of p53 and MMP-2 in lung carcinoma tissues was higher than those in control group (P〈0.05). Expressions of p53 and MMP-2 in lung carcinoma showed a positive correlation (t=-0.533 ,P〈0.01). [Conclusion] QDs-II-IC can be used to detect the expression of p53 and MMP-2. Expression of p53 and MMP-2 might play an important role in predicting the progress of lung carcinoma.
出处
《肿瘤学杂志》
CAS
2014年第6期456-459,共4页
Journal of Chinese Oncology