HPLC法同时测定运脾散浸膏中柚皮苷、橙皮苷和苍术素的含量

Simultaneous Determination of Naringin, Hesperidin and Atractylodin in Yun-Pi Powder Extract by HPLC

目的：HPLC法同时测定运脾散浸膏中柚皮苷、橙皮苷和苍术素的含量。方法：色谱柱：Thermo ODS-2（250 mm×4.6 mm，5μm）；流动相：甲醇-水梯度洗脱，0-5 min（30%-40% A），5-15 min（40%-50%A），15-20 min（50%-79% A），20-30 min（79%-100% A），30-45 min（100% A），45-55 min（100%-30%A），55-60 min（30% A）；柱温：30℃；检测波长：283 nm；流速：1 mL·min^-1。结果：柚皮苷、橙皮苷、苍术素线性范围分别为0.00916-0.13700μg（r=0.9999）、0.00622-0.09130μg（r=0.9998）、0.00358-0.05370μg（r=0.9999），平均回收率分别为103.51%、96.82%、97.65%，RSD分别为1.37%、1.41%、1.49%（n=6）。结论：本方法专属性强，重复性好，可用于运脾散浸膏中柚皮苷、橙皮苷和苍术素的含量测定。

This study was aimed to simultaneously determinate the content of naringin, hesperidin and atractylodin in Y un-Pi （YP） Powder extract by HPLC. A Thermo ODS-2 column （250 mm × 4.6 mm, 5 μm） was used for the gra-dient elution with a methanol-water of 0-5 min （30%-40% A）, 5-15 min （40%-50% A）, 15-20 min （50%-79%A）, 20-30 min （79%-100% A）, 30-45 min （100% A）, 45-55 min （100%-30% A）, 55-60 min （30% A）. The col-umn temperature was at 30℃. The detection wavelength was at 283 nm. The flow rate was 1 mL·min^-1. The linear range was determined to be 0.009 16-0.137 00 μg （r = 0.999 9）, 0.006 22-0.091 30 μg （r = 0.999 8）, 0.003 58-0.053 70 μg （r = 0.999 9）, respectively for naringin, hesperidin and atractylodin. The average recovery rate of pterodontic acid was 103.51%, 96.82% and 97.65%. And RSD was 1.37%, 1.41%, 1.49% （n = 6）. It was concluded that the method had good specificity and repeatability. It was considered as a good tool for the content determination of naringin, hesperidin and atractylodin in YP Powder extract.