洛阳地区献血员人类血小板抗原1～10频率分布调查

Investigation of frequency distribution of human platelet antigen-1to-10 in Luoyang

目的：研究洛阳地区献血员人类血小板抗原（HPA）1～10基因多态性，为患者提供HPA相配合的血小板。方法：随机收集250例无偿献血员样本，应用聚合酶链式反应一序列特异引物（PCR—SSP）技术检测HPA-1～10基因型及等位基因频率。结果：HPA-1aa、-1ab所占百分比分别为98．80％和1．20％，HPA-2aa、-2ab所占百分比分别为90．80％和9．20％，HPA-4aa、-4ab所占百分比分别为98．00％和2．00％，HPA-5aa、-5ab所占百分比分别为96．40％和3．60％，HPA-aa、-ab、-bb三种基因型在HPA-3和HPA-6中的比例分别为29．20％、46．00％、24．80％和91．60％、6．80％、1．60％，在HPA7、-8、-9、10中仅检测到HPA—aa纯合子。结论：洛阳地区HPA-1～HPA-10基因分布与文献报道相似，HPA-3特异性抗体是引起本地区HPA同种免疫性疾病的首要原因。

Objective：To study the gene polymorphism of human platelet antigen（HPA） -1 to 10,in order to provide patients with the HPA compatible platelets. Method：A total of 250 samples were collected from the blood donors randomly, and the genotypes and allele frequencies of HPA-1 to 10 were detected by polymerase chain reac- tion sequence specific primers （PCR-SSP）. Result：The percentages of HPA-laa and-lab were 98.80% and 1.20% respectively. The percentages of HPA-2aa and-2ab were 90.80% and 9.20% respectively. The percentages of HPA-4aa and-4ab were 98.00% and 2.00% respectively. The percentages of HPA-5aa and 5ab were 96. 40% and 3.60% respectively. The proportions of HPA aa,-ab and-hb in HPA-3 and HPA-6 were 29.20%, 46.00%, 24.80 % and 91.60 %, 6.80 %, 1.60 % respectively. The HPA-aa homozygous only was detected out in HPA-7 ,- 8,-9 ,-10. Conclusion： The gene distributions of HPA-1 to HPA-10 was similar to those reported in the literature. The HPA-3 specific antibody was the major cause for HPA autoimmune diseases in Luoyang.