IMP3与IGF2在乙肝相关性肝癌组织中表达及与临床病理特征的关系

Expression of IMP3 and IGF2 in hepatitis B-virus associated hepatocelluar carcinoma and the relationship with clinicopathological features

目的：研究胰岛素样生长因子2 mRNA结合蛋白3（IMP3）与胰岛素样生长因子2（IGF2）在乙肝相关性肝癌中的表达,并探讨二者的相关性及与乙肝相关性肝癌临床病理特征的关系。方法：采用免疫组织化学法,检测82例乙肝相关性肝癌组织及对应癌旁组织中IMP3及IGF2的表达;采用实时定量聚合酶链式反应法检测32例冰冻肝癌组织中IMP3及IGF2 mRNA含量;采用蛋白质印迹法检测4例IMP3强阳性染色及4例IMP3阴性染色对应的新鲜冰冻肝癌组织中IMP3及IGF2蛋白含量。结果：IMP3在62.2%肝癌组织中表达,在癌旁组织中不表达。IGF2在59.8%肝癌组织及14.6%癌旁组织中表达。同时,IMP3与IGF2在乙肝相关性肝癌组织中表达密切相关（rn = 0.335,P = 0.002）。另外,IMP3高表达与Edmondson-Steiner 3-4级（P = 0.001）、TNM 3-4期（P = 0.001）、α-甲胎蛋白（AFP）〉 200 ng/ml （P = 0.012）及伴有门脉癌栓的肿瘤（P = 0.018）均密切相关;与IMP3表达相似,IGF2高表达亦与Edmondson-Steiner 3-4级（P = 0.001）、TNM 3-4期（P 〈 0.001）及伴有门脉癌栓的肿瘤（P 〈 0.001）密切相关。结论：IMP3与IGF2在乙肝相关性肝癌组织中表达密切相关,同时,IMP3与IGF2蛋白分子的检测有助于判断肿瘤恶性分化程度及进展。

Objective：To investigate the expression of insulin-like growth factor 2 mRNA binding protein 3 （IMP3） and insulin-like growth factor 2 （IGF2） in hepatitis B-virus associated hepatocelluar carcinoma （HBV-HCC） and study the correlation between IMP3 and IGF2 as well as their relationship with clinicopathological features of HBV-HCC. Methods：IMP3 and IGF2 were detected in 82 paraffin-embedded tumor tissues and corresponding adjacent benign tissues from HBV-HCC patients by immunohistochemical assay. IMP3 and IGF2 mRNA were detected in selected 32 freezing tissues by Real-time PCR. IMP3 and IGF2 proteins were detected in 4 strong positive and 4 negative immunohistochemical staining tissues by Western blot analysis. Results：IMP3 was expressed in 62.2% HBV-HCC tissues,but not detected in adjacent benign tissues. IGF2 was expressed in 59.8% HBV-HCC tissues and 14.6% adjacent benign tissues. Meanwhile,the expression of IMP3 was significantly correlated with IGF2 expression in HBV-HCC tissues （rn = 0.335,P = 0.002）. Additionally,our results showed that IMP3 expression was significantly correlated with TNM 3-4 tumor stage （P = 0.001）,Edmondson-Steiner 3-4 tumor grade （P = 0.001）,Alpha Fetal Protein （AFP）〉 200 ng/ml （P = 0.012） and portal vein tumor thrombus （P = 0.018）. Similar to the expression of IMP3,IGF2 expression was significantly correlated with Edmondson-Steiner 3-4 tumor grade （P = 0.001）,TNM 3-4 tumor stage （P 〈 0.001） and portal vein tumor thrombus （P 〈 0.001）. Conclusion：Our results indicate that IMP3 was significantly correlated with IGF2 expression in HBV-HCC and detection of IMP3 and IGF2 was helpful to evaluate the differentiation and progression of HBV-HCC.