IL-13基因多态性与喉癌遗传易感性的关联研究

Association of genetic polymorphisms of IL-13 and risk of laryngeal cancer

目的：探讨白细胞介素-13（interleukin-13,IL-13）基因单核苷酸多态性（single-nucleotide polymorphism,SNP）与喉癌遗传易感性的关系。方法：通过病例-对照研究设计,以111例经病理确诊的喉癌患者和340例健康对照者为研究对象,选取IL-13基因3’-UTR的SNP位点rs1295685,采用TaqMan探针方法进行多态性检测,应用Logistic回归计算OR值及其95%CI,比较不同基因型与喉癌发病风险的关联。结果：病例组与对照组中rs1295685（IL-13C870T）位点基因型分布存在统计学差异,在调整年龄。吸烟。饮酒及肿瘤家族史因素后发现,与CC基因型相比,携带TT/CT基因型者喉癌发病风险显著增高（调整OR值为2.00,95%CI为1.23-3.27,P值为0.005）。与CC基因型相比,携带TT/CT基因型在年龄≤62岁、吸烟、饮酒、无肿瘤家族史各亚组中表现出更高的喉癌患病风险（OR = 2.72,95%CI：1.40-5.28; OR = 2.07,95%CI：1.19-3.58;OR = 1.96,95%CI：1.03-3.73;OR = 2.01,95%CI：1.18-3.41）。吸烟。饮酒均与TT/CT基因型存在协同作用（OR = 6.48,95%CI：2.77-15.14和OR = 7.35,95%CI：3.15-17.12）。而基因型分布在肿瘤分期、分级中未见显著差异。结论：IL-13基因rs1295685位点的单核苷酸多态性与喉癌遗传易感性有关联。

Objective：To investigate the association between single nucleotide polymorphisms of IL-13 gene and susceptibility to laryngeal cancer in a Chinese population. Methods：A case-control study included 111 laryngeal squamous cell carcinoma （LSCC） patients and 340 cancer-free controls. Genotyping of the polymorphisms located at the 3’untranslated region （UTR） of IL-13 gene was conducted using MGB TaqMan Probe Assay. Logistic regression model was used to assess the contribution of genetic effects on the development of laryngeal cancer. Results：Individuals with IL-13 rs1295685 TT/CT genotypes had a increased risk of laryngeal cancer （adjusted OR = 2.00,95%CI：1.23-3.27,P = 0.005）. Particularly among younger patients （OR = 2.72 and 95%CI：1.40-5.28）,smokers （OR = 2.07 and 95%CI：1.19-3.58）,drinkers （OR = 1.96 and 95%CI：1.03-3.73） and those without a family history of cancer （OR = 2.01 and 95%CI：1.18-3.41）. Furthermore,compared with those,who were both nonsmokers and CC carriers,significantly increased risk was observed in those,who were smokers and TT/CT carriers （OR = 6.48 and 95%CI：2.77-15.14）. The similar situation was found in drinkers（OR = 7.35 and 95%CI：3.15-17.12）. No significant relationship was observed between rs1295685 polymorphism and progression of laryngeal cancer. Conclusion：The polymorphisms of IL-13 gene may play an important role in the etiology of laryngeal cancer.