摘要
目的:利用细胞内脱氢酶对2,3,5-三苯基氯化四氮唑(TTC)的催化作用建立比目前常用的3-(4,5-二甲基-2-噻唑)-2,5-二苯基溴化四氮唑(噻唑蓝,MTT)法更可靠的测定细胞活力的方法。方法使用不同浓度的TTC溶液测定细胞活力,并确定TTC的有效浓度范围。在计算所测定值与细胞数量函数关系的基础上,通过与传统测定细胞活力的MTT法进行比较,分析该方法的可行性。结果0.5%~1.0%浓度范围内的TTC溶液可用来测定细胞活力,测定值与细胞数量之间存在线性关系,与MTT法得到的结果基本一致,可避免MTT法测定过程中来自于细胞培养体系中其他抗氧化物质的非特异性干扰。结论 TTC可用于测定细胞活力,可替代常规的MTT法,具有操作简便,费用经济,结果可靠等优点。
Objective To develop a precise and reliable method to determine mammalian cell survival using the triphe-nyltetrazolium chloride (TTC) solution.Methods Different amounts of TTC solution were added to the cultured cells in order to find the optimal concentration .The correlation between the cell number and the measured optic density value was analyzed.In addition, the TTC method was compared with traditional MTT assay .Results The TTC assay could precisely reveal the viability of cultured cells as well as the MTT method , but the non-specific reaction of the MTT reagent by other antioxidants could result in unreliable overestimation of the cell number .Conclusion The TTC assay can be used to deter-mine the survival of cultured cells more precisely .
出处
《军事医学》
CAS
CSCD
北大核心
2014年第5期388-391,共4页
Military Medical Sciences
基金
国家重点基础研究发展计划(973计划)资助项目(2012CB518200)
国家自然科学基金资助项目(81271206
81100979
31101049
81070741)
国家科技重大专项"十二五"计划资助项目(2012ZX09102301-016)
蛋白质组学国家重点实验室资助项目(SKLP-K201004
SKLP-O201104)