摘要
目的了解沈阳周边山区林区蜱携带新型布尼亚病毒的情况,为沈阳地区发热伴血小板减少综合征的防控提供依据。方法应用蜱种鉴定引物和新型布尼亚病毒特异性引物,采用巢式反转录-聚合酶链反应方法,对2012年4-9月在沈阳周边马耳山、棋盘山和内蒙古与沈阳康平交界地区农户家养动物体表采集的蜱标本1989只进行检测,对扩增得到的目的条带进行基因测序并与GenBank中注册的基因序列进行同源性比较。结果通过对采集的蜱标本进行测序和同源性比较,蜱种鉴定结果为长角血蜱,新型布尼亚病毒检测结果均为阴性。结论目前从沈阳周边山区林区采集的蜱主要为长角血蜱,蜱中尚未发现新型布尼亚病毒的存在,沈阳地区是否存在该病毒的自然疫源地尚有待进一步的深入调查研究,针对新型布尼亚病毒应建立定期监测机制。
Objective To understand the carriage of novel Bunyavirus in ticks in mountain and forest areas around Shenyang and provide scientific evidence for the prevention of fever with thrombocytopenia syndrome in Shenyang. Methods RT-PCR was performed to detect 1989 ticks collected in Maer mountain, Qipan mountain and the area bordering Inner Mongolia from April to September 2012. Results By sequence analysis and homologous comparison, it was conclude that the ticks detected in Shenyang belonged to Haemaphysalis longicornis and was negative for novel Bunyavirus. Conclusion The ticks in mountain and forest areas around Shenyang were Haemaphysalis longicornis which were negative for Bunyavirus. Further study is needed to determine whether the natural focus of novel Bunyavirus exists in Shengyang or not and novel Bunyavirus surveillance system should be established too.
出处
《疾病监测》
CAS
2014年第5期395-398,共4页
Disease Surveillance
