期刊文献+

高氧对新生小鼠视网膜谷氨酸释放的影响 被引量:1

Effectand Mechanism of Glutamate Release at Hyperxia Induced Retinal Injury in Newborn Mice
下载PDF
导出
摘要 为研究新生小鼠高氧状态下视网膜谷氨酸摩尔质量浓度的变化及其机制,探讨谷氨酸在高氧视网膜损伤中的作用,实验组将新生小鼠置于体积分数为95%的高氧环境中造成视网膜损伤,对照组将新生小鼠置于正常空气中,HE染色观察视网膜损伤情况,多功能酶标仪检测视网膜谷氨酸(Glu)摩尔质量浓度变化,逆转录聚合酶链反应(RT-PCR)检测视网膜谷氨酸转运体(GLAST)与谷氨酰胺合成酶(GS)mRNA表达情况.结果发现,与空气对照组比较,高氧处理组视网膜受损,视网膜Glu摩尔质量浓度增加,GLAST和GS mRNA表达下降(P<0.05).高氧可导致新生小鼠视网膜的谷氨酸增高,其机制与GLAST和GS mRNA表达下降有关.谷氨酸增加可能是高氧导致视网膜损伤的原因之一. The concentration change and mechanism of glutamate at hyperxia induced retinal injury in newborn mice retinal injury are explored. The experimental group was put in 95 % hyperxia environment to induce retinal damage,and the control groups in normal air. HE staining was used to observe retinal injury,ELIASA to detecte the glutamate (Glu) concentration,and reverse transcription polymerase chain reaction(RT-PCR) to analyze the expression of retinal glutamate transporter (GLAST) and glutamine synthetase (GS) mRNA. The results showed that, compared with control group, hyperxia treatment group with retinal ifijury showed the increased Glucontent,and decreased GLAST and GS mRNA expression. It thus can be concluded that hyperxia can lead to the increase of retinal glutamate in newborn mice, the mechanism of which was associated with decreased GLAST and GS mRNA expression. Glutamate increase may be one of the reasons for the hyperxia induced retinal injury.
出处 《吉首大学学报(自然科学版)》 CAS 2014年第2期90-93,共4页 Journal of Jishou University(Natural Sciences Edition)
关键词 高氧 谷氨酸 视网膜 新生 小鼠 hyperxia glutamate retina newborn mice
  • 相关文献

参考文献6

二级参考文献64

共引文献37

同被引文献4

引证文献1

二级引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部