雷竹不同栽培类型RAPD分子标记的研究

Studies on cultivars of  Phyllostachys pxaecox with RAPD molecular markers

对雷竹的 1 9个栽培类型及 2个近缘种进行了RAPD分析。从 4组 80个引物中筛选出1 0个引物 ,产生随机扩增位点 66个 ,其中多态性位点 54个 (占 81 8% )。利用POPGEN 32程序进行聚类分析 ,结果表明 :①聚类结果可将雷竹的不同栽培类型及其近缘种区分开来 ,以前认为是雷竹一栽培型的永嘉雷竹不属于Ph .praecox种系 ;②细叶雷竹与阔叶雷竹的产量高低主要取决于经营管理 ;③RAPD分子标记对竹类植物种以下等级进行分类有一定的可靠性。图 2表 3参

With RAPD molecular markers,19 cultivars and 2 affinis species of Phyllostachys praecox were analysed.Using 10 primers screened from 80 primers,66 loci were amplified,and 54 of these were polymorphic(81 8%).The UPGMA cluster analysis of POPGEN 32 program showed:(1)The cultivars and affinis species of Ph.?praecox can be divided by UPGMA analysis,and the Yongjia Lei Bamboo recognized as one of the cultivars of Lei Bamboo before doesnt belong to  Ph.?praecox.(2)The production of narrow leaved Lei Bamboo and broad leaved Lei Bamboo mainly decided by management.(3)The classification on bamboo under species based on RAPD markers is somewhat reliable.