喜树叶总黄酮优化提取方案的研究

Optimum scheme of extracting flavonoid in Camptotheca acuminata leaves

采用二次通用旋转组合设计方法 ,用pH =1 0的水作为溶剂 ,在不同的提取温度、溶剂体积和提取时间的条件下对喜树叶总黄酮的含量进行提取试验 ,建立三元二次回归方程并进行分析。对三因素的不同水平搭配组合 ,应用计算机进行模拟试验得到 93=72 9个数据 ,由此推求最优提取方案。结果表明 ,以pH =1 0的水为溶剂 ,提取温度 91～ 1 0 0℃ ,溶剂体积 50～ 60mL ,提取时间 2 5～ 37min ,可最大限度地提取喜树叶中的黄酮。表 3参

Under the different temperatures,solvent volumes and extracting hours,using water (pH=10)as solvent,the experiment how to more extract content of flavonoid in Camptotheca acuminata leaves was made by use of quadratic current spinning combination design metod.According to the experimental data,an ternary quadratic form regression equation was built and analyzed;and a simulate test to different leaves of three factors was did with computer.The results showed that the optimum scheme of extracting flavonoid from Camptotheca acuminata leaves was extracting temperature of 90-100 ℃,solvent volume of 50-60 mL and extracting time of 25-37 min.