大豆总DNA直接导入法培育优质高蛋白玉米材料研究

A study on the screening high-protein variants by transmitting exogenous soybean DNAs into maize

利用外源总DNA转导的花粉管通道法和经我们发展的减压渗透法将大豆总DNA导入玉米自交系统 4 8- 2和S3 7,在 74 3份后代材料中筛选出 8份高蛋白含量变异材料 ,它们的蛋白质与受体相比提高 2 0 %以上。用种子蛋白SDS -PAGE、谷氨酸 -草酰乙酸同工酶及RAPD等分析方法对变异材料及其受体进行了鉴定分析 ,同时也对此 8份变异材料的自交后代进行了筛选和在田间比较观察了变异材料与受体的农艺性状。结果表明 ,这些高蛋白变异材料与受体 4 8- 2和S3 7在遗传上存在明显的差异 ,在其自交后代中仍有部分株系保持了高蛋白含量特性。此外 ,4 8- 2的变异材料在植株形态、花药颜色上有明显变化。上述结果初步证明高蛋白特性是可遗传的 ,这为选育高蛋白玉米新自交系提供了新材料 。

Eight high-protein variants were selected from 743 materials obtained by means of transmitting the soybean DNA into maize inbred lines 48-2 and S 37 with pollen tube pathway technique and decompressed permeation.Through analyzing their seed protein,screening their inbreed progeny in laborators by means of seed protein SDS-PAGE,GOT isozyme and RAPD,it was found that there was greatly genetic diversity between the high-protein variants and receptors.At same time,the variants in plant pattern and anther color were significantly different from receptors.The results indicated that the character of high-protein was hereditary,It can not only provide new materials for breeding high-protein maize inbreds lines,but also open a new effective way to use exogenous DNA introduction technique to create new maize material. -