摘要
利用农杆菌共培养法将雄性不育基因 (TA2 9 Barnase)导入水稻品种日本晴的胚性愈伤。通过 50～ 150mg/L卡那霉素筛选 ,从 50 0个胚性愈伤中获得 16个筛选愈伤团 ,并分化出了3株绿苗。经PCR扩增分析 ,16个筛选愈伤团中有 7个呈阳性 ;
The male sterile gene-TA29-Barnase was introduced into rice embryo calli by Agrobaterium tumefaciens transformation.Resistant calli were selected on Kan(50 to 150 mg/l)medium,Three green plants were generated from sixteen resistant calli derived from 500 embryo calli and calli and plants are analysed by PCR.
出处
《西南农业学报》
CSCD
2001年第1期13-15,共3页
Southwest China Journal of Agricultural Sciences
基金
云南省应用基础研究基金资助项目
关键词
胚性愈伤
水稻
基因转化
雄性不育基因
农杆菌共培养法
TA29-Barnase gene
Agrobaterium tumefaciens
embryo calli:transgenic rice plant
co-cultivation
