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食用菌种质资源保藏方法研究 被引量:4

Studies on the conserving methods of edible fungus strain resources
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摘要 通过对三种封口方式保藏管的基质形态和菌丝回接成活率比较 ,证实有孔胶塞是一种较理想的封口方式 ,它能协调保水与通气的矛盾。对琼脂斜面低温保藏、自然基质柱状低温保藏和液体石蜡常温保藏比较表明 ,前两者结合使用 ,是一种简单易行的短。 The configuration of culture media and surviving rate of mycelium returning inoculation were compared by three sealing up test tube manners of conserving tube.The result showed that gum stopper with hole was a relatively perfect sealing up way.It could coordinate the contravention between keeping water and aerating.This paper also studied the conserving tube peculiarities in agar-slant low temperature,nature culture media pole shape low temperature and liquid olefin normal temperature,respectively.The result indicated that former both combined to use was a simple and plausible method to conserve strains in long and short term. -
出处 《西南农业学报》 CSCD 2001年第1期96-98,共3页 Southwest China Journal of Agricultural Sciences
关键词 琼脂斜面 自然基质 液体石蜡 保藏方法 封口方式 食用菌 种质资源 strain agar-slant nature culture liquid olefin conserving method
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