摘要
以羧甲基纤维素钠为碳源,从牛瘤胃中分离出382株能降解纤维素的细菌菌株,经初筛、复筛证明,C5菌株分解纤维素能力最强。通过测定不同培养条件下C5菌株的产酶能力,初步确定其最佳产酶条件为:最适pH值6.0,最适温度28℃,最适摇床转速180 r/min,培养64 h后酶活力达到高峰。
382 strains of bacteria degrading cellulose from cattle rumens taking the CMC-Na as solecarbon resource. For these strains, the highest C5 stain was gained by primary screening and second-ary screening. The optimum conditions for cellulase production by C5 strain were studied. The resultsshowed that C5 strain had a strong ability to degrade cellulose. And the results show that the optimumcultural temperature is 28 ℃, the original pH value 6.0, shaking speed 180 r/min and the suitable cul-ture time is 64 h. In the condition, the enzyme activity reached the peak.
出处
《饲料工业》
北大核心
2014年第12期45-47,共3页
Feed Industry
关键词
纤维素
降解菌
筛选
酶活力
cellulose
degradative strain
screening
enzyme activity
