摘要
采用普通水提法、高温高压水提法、冷碱法、热碱法对槐耳(Trametes robiniophila)子实体进行提取,考察了水溶性提取物中粗多糖含量、粗多糖实际得率和对K562细胞的抑制率。碱提法水溶性提取物的得率高于水提法,其中热碱法提取物的得率最高为29.65%,但其粗多糖含量最低,冷碱法粗多糖的实际得率最高为6.93%。四种提取物对K562细胞均有抑制作用,热碱法提取物浓度为2mg/mL作用于K562细胞72h后,抑制率高达92.28%。
Crude polysaccharide was extracted from Trametes robiniophila fruit bodies at different temperatures using either water or alkali.The highest extract yield (29.65%)was recorded using NaOH at 65 ℃ but this extract contained the lowest crude polysaccharide content.Highest crude polysaccharide yields (6.93%)were recorded in samples extracted with alkali at 4 ℃.All extracts inhibited the growth of K562 cells,with the highest inhibition rate (92.28%)recorded following treatment of the cells for 72 h with 2 mg/mL concentrations of material extracted with NaOH at 65 ℃.
出处
《食用菌学报》
北大核心
2014年第1期55-58,共4页
Acta Edulis Fungi
基金
国家自然科学基金资助项目(编号:31172012)的部分研究内容
关键词
槐耳
提取物
提取方法
粗多糖
K562
抑制率
Trametes robiniophila
extraction method
crude polysaccharide
inhibition of K562 cell growth