不同提取方法对槐耳提取物的粗多糖含量及K562细胞抑制作用的影响

Crude Polysaccharide Extraction from Trametes robiniophila Fruit Bodies and Inhibitory Effects on the Growth of K562 Cells

采用普通水提法、高温高压水提法、冷碱法、热碱法对槐耳(Trametes robiniophila)子实体进行提取,考察了水溶性提取物中粗多糖含量、粗多糖实际得率和对K562细胞的抑制率。碱提法水溶性提取物的得率高于水提法,其中热碱法提取物的得率最高为29.65%,但其粗多糖含量最低,冷碱法粗多糖的实际得率最高为6.93%。四种提取物对K562细胞均有抑制作用,热碱法提取物浓度为2mg/mL作用于K562细胞72h后,抑制率高达92.28%。

Crude polysaccharide was extracted from Trametes robiniophila fruit bodies at different temperatures using either water or alkali.The highest extract yield （29.65%）was recorded using NaOH at 65 ℃ but this extract contained the lowest crude polysaccharide content.Highest crude polysaccharide yields （6.93%）were recorded in samples extracted with alkali at 4 ℃.All extracts inhibited the growth of K562 cells,with the highest inhibition rate （92.28%）recorded following treatment of the cells for 72 h with 2 mg/mL concentrations of material extracted with NaOH at 65 ℃.