人工培养蛹虫草核苷类HPLC指纹图谱的建立

Nucleoside Fingerprinting of Cultured Cordyceps militaris Strains Using HPLC

建立了蛹虫草(Cordyceps militaris)核苷类高效液相色谱(HPLC)指纹图谱的分析方法,采集10批不同产地人工培养蛹虫草HPLC图谱,运用"中药色谱指纹图谱相似度评价系统2004A版"软件建立人工蛹虫草标准图谱并以该模式为参照,计算10批不同产地蛹虫草之间的相似度。结果表明:方法的稳定性、精密度、重现性符合方法学要求;建立的核苷类HPLC指纹图谱共得到10个共有峰,并对确认了虫草素和腺苷峰这两个特征峰,各样品图谱与标准指纹图谱的相似度均在0.9以上。

A stable,precise and reproducible HPLC-based method for determining the nucleoside fingerprints of cultured Cordyceps militaris strains has been developed.Extracts were prepared by suspending 0.5 g powdered fruit body in 20 mL 20% methanol and exposing to ultrasound （40 kHz）for 1 h at 30 ℃.After filtration,samples were subjected to HPLC for nucleosides analysis. HPLC spectra obtained from ten cultured C.militaris fruit body samples were subjected to professional analytical software recommended by the Pharmacopoeia Commission of People’s Republic of China （China version 2004A）to create a standard HPLC fingerprint for cultured C.militaris consisting of ten common peaks.Cordycepin and adenosine peaks in the fingerprint were confirmed by comparing the retention times with those of cordycepin and adenosine standards.Similarity coefficients between C.militaris samples and the fingerprint standard were all higher than 0.9 .