野生地木耳中次生代谢产物含量测定与分析

Quantitative analysis of plant secondary metabolites in wild Nostoc commune Vauch.

测定了野生地木耳（Nostoc commune Vauch.）中次生代谢产物含量,并与4种常见食用菌藻黑木耳（Auricularia auricular-judae）、银耳（Tremella fuciformis）、紫菜（Porphyra）、海带（Laminaria japonica）进行比较分析,总酚、总黄酮和缩合单宁含量测定采用分光光度法,总生物碱含量测定采用高效液相色谱法（HPLC）。结果显示,野生地木耳中总酚含量为24.255 mg/g±1.631 mg/g,总黄酮含量为5.741 mg/g±0.239 mg/g,总生物碱含量为0.768 mg/g±0.073 mg/g,缩合单宁含量为0.069 mg/g±0.009 mg/g。4种食用菌藻中次生代谢产物含量范围为：总酚（5.520～62.326 mg/g）、总黄酮（0.847～7.010 mg/g）、总生物碱（0.408～4.132 mg/g）、缩合单宁（0.063～0.233 mg/g）;比较分析结果显示,野生地木耳次生代谢产物中的总酚和总黄酮物质含量较高,且总酚是主要次生代谢产物,缩合单宁与总生物碱含量较低;总酚含量约为黑木耳和银耳的2倍、紫菜的6倍;总黄酮含量约为黑木耳的7倍,银耳的3倍。

This experiment determined the contents of plant secondary metabolites（ PSMs） in wild Nostoc commune Vauch.,and analyzed the differences of the PSMs contents among wild Nostoc commune Vauch.,Auricularia auricular-judae,Tremella fuciformis,Porphyra and Laminaria japonica. The contents of condensed tannins,total polyphenol and flavonoids in wild Nostoc commune Vauch.were tested by UV-VIS spectrophotometer,and the contents of total alkaloids were quantified by high performance liquid chromatography（ HPLC）. The results showed that the content of total polyphenol was 24. 255 mg /g ± 1. 631 mg /g,the total flavonoids was 5. 741 mg /g ± 0. 239 mg /g,the total alkaloids was 0. 768 mg /g ± 0. 073 mg /g and the condensed tannins was 0. 069 mg /g ± 0. 009 mg /g in air-dry of wild Nostoc commune Vauch. The content range of several kinds of PSMs in daily edible fungoids and algaes was as follows：total polyphenol,5. 520 ～ 62. 326 mg /g; total flavonoids,0. 847 ～ 7. 010 mg /g; total alkaloids,0. 408 ～ 4. 132 mg /g; and condensed tannins,0. 063 ～ 0. 233 mg /g. The contents of total polyphenol and flavonoids are more abundant than those in daily edible fungoids and algaes,and the contents of condensed tannins and total alkaloids are lower in wild Nostoc commune Vauch.