超临界CO_2萃取烟草精油的工艺研究

Study on the Extraction Process of Essential Oil from Tobacco by Supercritical CO_2 Fluid

以烟草为材料,以精油提取率、新植二烯提取率、精油品质为评价指标,通过单因素实验考察夹带剂乙醇的体积分数、夹带剂流量、萃取压力、萃取温度、CO2流量、萃取时间等因素对萃取效果的影响。在此基础上,通过正交实验优选萃取工艺条件为:94%乙醇为夹带剂、夹带剂流量0.04mL·min-1、萃取压力25MPa、萃取温度50℃、CO2流量2.0L·min-1、萃取时间3.0h,在此条件下萃取的精油金黄透亮,夹带剂残留少,精油提取率为37.58mg·g-1,新植二烯提取率为4.045mg·g-1。

Using tobacco as raw material, with the extraction rate of essential oil and neophytadiene, the quality of essential oil as evaluation indexes, the effects of volume fraction and flux of modifier, extraction pressure, extraction temperature, supercritical CO2 fluid flux, extraction time on extraction efficiency were investigated by single factor experiment. Based on the results obtained,the optimum process parameters were determined by orthogonal experiment as follows：with 94% ethanol at the flux of 0.04 mL·min^-1 as modifier,extraction pressure was 25 MPa,extraction temperature was 50 ℃,supercritical CO2 fluid flux was 2. 0 L·min^-1,extraction time was 3.0 h. Under the optimal conditions,the essential oil was golden in color and had fewer residues, the extraction rate of essential oil and neophytadiene was 37.58 mg ·g^-1 and 4. 045 mg ·g^-1 respectively.