痰消化集菌法用于结核分枝杆菌检测的可行性研究

Feasibility Study of Digestion and Collection Method in Detection of Mycobacterium Tuberculosis in Sputa Samples

目的探讨痰消化集菌法进行痰标本结核分枝杆菌检测的临床价值。方法收集2012年3—10月门诊和住院疑似肺结核225例的痰标本,分别采用直接涂片法和消化集菌法检测结核分枝杆菌并比较。结果 225例痰标本采用消化集菌法检测结核分枝杆菌阳性率为38.67%,直接涂片法25.78%,消化集菌法检出阳性率高于直接涂片法(P<0.05)。镜下检出痰液标本结核分枝杆菌不同数量时的阳性率消化集菌法明显高于直接涂片法,尤以结核分枝杆菌在(++++)时最明显,此时消化集菌法阳性率35.63%,直接涂片法12.07%,组间比较差异有统计学意义(P<0.05)。结论痰消化集菌法用于痰标本结核分枝杆菌检测具有操作简便、检出阳性率高及实验室安全等优点。

Objective To explore the clinical value of digestion and collection method in detection of mycobacterium tuberculosis in sputa samples. Methods A total of 225 sputa samples of patients suspected as having tuberculosis from March to October 2012 were collected,and mycobacterium tuberculosis was detected with digestion and collecting method and direct smear method respectively,and then the results of the two methods were compared. Results The positive rates of 225 sputa samples were 38. 67% by digestion and collection method and 25. 78% by direct smear method respectively,and the positive rate by digestion and collection method was significant higher than that by direct smear method（ P 〈0. 05）. The positive rates of mycobacterium tuberculosis in different quantities detected under microscope were significantly higher than those by digestion and collection method,especially when the quantity of mycobacterium tuberculosis was at（ ＋ ＋ ＋ ＋）,and at the moment the positive rate of digestion and collection method was 35. 63%,while the positive rate of that direct smear method was 12. 07%,and the differences between the groups were statistically significant（ P 〈0. 05）. Conclusion Digestion and collection method in detection of mycobacterium tuberculosis in sputa samples is safe and simple with a higher positive detection rate.