乙肝病毒HBx-d382与HBx-d431突变体特异性抗体检测方法的建立及临床应用

Development of Method for Detection of Specific Antibodies of Hepatitis B Virus X Gene Deletion Mutants HBx-d382 and HBx-d431 and Evaluation of its Clinical Use

目的建立检测乙肝病毒X基因突变体HBx-d382和HBx-d431特异性抗体的方法,探讨其在诊断HBV相关性肝细胞癌中的应用价值。方法通过生物信息和多肽合成的方法,制备HBx-d382和HBx-d431特异性多肽抗原。采用间接ELISA法对正常人和慢性乙肝病毒感染者和肝细胞癌病人血清中抗HBx-d382和抗HBxd431特异性抗体进行检测。结果肝细胞癌病人血清中抗HBx-d382和抗HBx-d431抗体的阳性率均高于正常人、慢性乙肝病毒感染者(P<0.05),慢性乙肝病毒感染者抗HBx-d382和抗HBx-d431抗体的阳性率高于正常人(P<0.05)。慢性乙肝病毒感染者HBsAg(+)/HBeAb(+)/HBcAb(+)模式抗HBx-d382阳性率明显低于HBsAg(+)/HBeAg(+)/HBcAb(+)模式和HBsAg(+)/HBcAb(+)模式(P<0.05),而抗HBx-d431和抗HBxP阳性率在这些血清学模式之间无显著性差异(P>0.05)。结论抗HBx-d382和抗HBx-d431是乙肝病毒感染的一种特异性抗体,可以用于HBV携带者及慢性乙肝病人的监测,对预测肝细胞癌的发生有一定的意义。

Objective To develop the method for detection of specific antibodies of hepatitis B virus X gene deletion mutants HBx-d382 and HBx-d431 and study its clinical value in the diagnosis of HBV-related hepatocellular carcinoma. hepatitis B virus mutants HBx-d382 and HBx-d431,and it is significant for early diagnosis of HBV-related hepatocellular carcinoma,HBV carriers and chronic hepatitis B patient monitoring.