萘乙酸对董棕变温冷胁迫的生理效应

Effects of NAA on physiological indexes of Caryota obtusa seedlings under variable temperature stress

为了研究不同浓度萘乙酸(NAA)溶液对董棕(Caryota obtusa)幼苗耐寒性的影响,分别用0,25,50和100 mg·L-14种浓度的NAA溶液喷施董棕叶片,并分别经过25℃—5℃—25℃各24 h变温冷胁迫处理后,研究董棕叶片在变温冷胁迫过程中超氧化物歧化酶(SOD)、过氧化物酶(POD)、丙二醛(MDA)、脯氨酸、可溶性蛋白和叶绿素含量等生理指标的变化规律,并对25℃恢复24 h后的董棕幼苗上述耐寒性指标进行模糊隶属函数综合评价,结果表明:4种浓度下隶属函数值分别为0.39,0.30,0.39和0.51,其中喷施100 mg·L-1的NAA溶液对提高董棕的耐寒性效果最好。

The effects of naphthalene acetic acid（ NAA） on the cold tolerance of Caryota obtusa seedlings were conducted by several physiological indexes,The leaves of C. obtusa were sprayed with 0,25,50 and 100 mg·L^-1NAA solutions, the activities of superoxide dismutase（ SOD）, peroxidase（ POD）, and the contents of malondialdehyde（ MDA）,proline,soluble protein and chlorophyll of C. obtusa were determined under 25℃,5℃and 25℃. It was indicated that all the above physiological indexes varied with different concentrations of NAA.Subordinate function values for different concentrations of NAA at 25℃ were 0. 39,0. 30,0. 39 and 0. 51,respectively. It was concluded that leaves sprayed with different concentrations of NAA could affect the cold tolerance of C. obtusa seedlings and the optimal concentration was 100 mg·L^-1.