大鼠血液中溴莫普林的代谢产物核磁共振氢谱和质谱研究

Study on the Metabolites of Brodimoprim in Rat Plasma with^1H Nuclear Magnetic Resonance and Mass Spectroscopy

用核磁共振氢谱和质谱法研究大鼠血浆中溴莫普林 (BDP)及其代谢物。首先用固相萃取法将血液中内源性物质除去 ,然后经核磁共振氢谱和质谱检测大鼠血液中BDP及其代谢物。大鼠服用溴莫普林后 2 0h时血浆中检测到两个代谢产物 :脱甲基 溴莫普林葡萄糖醛酸和溴莫普林硫酸。结果证明核磁共振氢谱和质谱法可以快速、方便地用于血液中药物及其代谢产物的含量与结构检测的研究。

The methods of 1H Nuclear Magnetic Resonance (NMR) and mass spectroscopy were used in detecting the metabolites of brodimoprim (BDP) in rat plasma. The endogenic compounds in the plasma were removed with solid phase extraction (SPE) column firstly, then the mixture of metabolites was identified with 1H NMR and mass spectroscopy (MS). Two metabolites of BDP in the plasma at 20h were detected, they were demethyl-brodimorpim glucuronide and brodimoprim sulfurate. The study proved that the method of SPE coupled with NMR and MS can be applied to the analysis of metbolites in plasma quickly and conveniently.